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COMPARATIVE ANALYSIS OF MICROBIAL LOAD OF THE ENUGU MAIN WATER PRODUCTION AND WATER AVAILABLE TO IMT CAMPUS II HOSTEL

 

ABSTRACT

          Samples of water from the different hostels in campus II and other source of water production namely Ajali-owa water, from bore hole 9th mile and spring water from GRA water board were water collecterd and analysed using total plate count and multiple tube fermentaton techniques coliform.  The results shows no coliforms in production water from Ajali-owa and in GRA spring water reservaiour and no coliform in 9th mile bore hole water, 14 organism and 149 cfu/ml plate counts were obtained from tap water in IMT campus II hostel 7 coliform and Tocful / ml plate count from well water and 28 coliform and 280 cfu/ml plate count from spillage waste water.  These results obtained show that tap water  consumed in IMT campus II did not meet with NAFDAC and WHO standards.  Preventive measures such as proper treatment of these water should be encourage and all pipes in campus II should be checked for leakages

 

TABLE OF CONTENT

 

Title page                                                            

Certification                                                                  

Dedication                                                           

Acknowledgement                                               

Abstract                                                              

Table of content  

                                      

         

1.0     CHAPTER ONE

 Introduction                 

1.1     Aims / objectives of the study          

1.2     Statement of problems                     

1.3     Hypothesis                                                

1.4     Scope of limitation                 

 

 

                            

CHAPTER TWO

Literature review 

  1. The major group of biological Pollutions of water                                    

2.2     Source of water supply                    

2.3     Source of water pollution                          

2.4     Treatment of water sample              

  1. International standard for drinking water                                        
  2. Diseases from water     

                            

CHAPTER THREE

3.0     Materials & methods

3.0     Materials                                                   

3.1     Media preparation                                     

3.2     Collection of samples                                

3.3     Collection of samples                                

3.4     Methods of water analysis                         

3.5     Determination of PH                                 

  1. Determination of bacteria of the coliform                                        
  2. Culture techniques                                     
  3. Plate count technique                                 
  4. Presumption coliform (multiple tube fermebtation                 

3.5.4  Confirmatory test                            

3.5.5  Completed test                                          

3.5.6  Test for indole                                           

3.5.7  Methyl red test                                          

3.5.8  Sugar test                                                  

3.5.9  Oxidase test                                                        

3.5.10 Catakase test                                            

3.5.11 The slide cougulase test                            

3.5.12 Gram stain techniques                    

3.5.13 Detection of faecal stredtococci      

3.5.14 Detection of escherichi coli test      

3.5.15 Organism suspected after gram staining                                                            

CHAPTER FOUR

RESULTS                     

CHAPTER FIVE

Discussion

 

CHAPTER SIX

6.1     Conclusion

6.2     Recommendation

References

Appendix

 

 

CHAPTER ONE

 

INTRODUCTION

          Water is a liquid mineral.  It consist of two atoms of hydrogen combined with one atom of oxygen. Water is a tasteless, odourless and colourless mobile liquid except in large volume where it appears blue.  It has a melting point of OoC (320f) and a boiling point of 1000C (2120f).  water is a basic necessity of life, in the absence higher animals survive only but a few hours or days.  It has several uses such  as  washing  cooking, food  processing ,swimming, and  among  others. Out  of   these  uses, drinking  of  water  seems  to  be  the  most   sensitive  as  it  could  have  a  direct   deleterious  impact  on  health  of  human  beings.

          Therefore, drinking  water  should  be  potable  free  diseases, or  toxic  substance (Beren 1991) .

          Water is portable when it is colourless.  Odourless, or tasteless and also free from poisonous, corroding, staining substances as well as disease casuiong oirganisms (Cruich 1972).  The problem of providing safe and adequate water is as old as life on earth.  Water can be made unifit or unsafe for drinking it contamination occurs either by nature or activities of human beings.    

          Perhaps the greatest danger associated with drinking water is contamination by human  excrement (Forest 1979).  In most cases, the pollution/contamination is hardly severe and is not particularly detrimental to health.

          Thus a few substances and microbes that are health hazard do occur in water, and can cause illness or even death.  From microbiological point of view, the pathogens most frequently transmitted through water supply ranges from ultrasmall virus to the microspic bacteria and relatively large cysts entemoeba instolytica.  These organism cense infectrons of intestinal trach, when consumed censing amebic dysentery, and Gardia lamblia is a protozoa responsible for Giadiasis.  Defective plumbing for example was the cause of out break during the world fair in Chicago 1933 (Garelick 1987) water borne disease cause acute diarrhoe, often last 2-3 months.  The most common water borne disease can be by bacteria includes typhoid fever, paratyphoid.  Asiatic cholera and bacteria dysentery.

 

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